Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A
نویسندگان
چکیده
منابع مشابه
Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A.
Two proteins, a 56-kDa protein (p56) and a 58-kDa protein (p58), are produced from the hepatitis C virus (HCV) nonstructural region 5A (NS5A). Recently, we found that both proteins are phosphorylated at serine residues and that p58 is a hyperphosphorylated form of p56. Furthermore, hyper-phosphorylation depends on the production of an intact form of the HCV NS4A protein. To clarify the nature o...
متن کاملHepatitis C virus nonstructural protein 5A (NS5A) is an RNA-binding protein.
Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) has been shown to antagonize numerous cellular pathways, including the antiviral interferon-alpha response. However, the capacity of this protein to interact with the viral polymerase suggests a more direct role for NS5A in genome replication. In this study, we employed two bacterially expressed, soluble derivatives of NS5A to probe for no...
متن کاملThe N-terminal region of hepatitis C virus-encoded NS5A is important for NS4A-dependent phosphorylation.
We previously showed that two proteins, a 56-kDa protein (p56) and a 58-kDa protein (p58), are produced from the hepatitis C virus (HCV) nonstructural 5A region (NS5A) and that the production of p58 is enhanced by the presence of NS4A (T. Kaneko, Y. Tanji, S. Satoh, M. Hijikata, S. Asabe, K. Kimura, and K. Shimotohno, Biochem. Biophys. Res. Commun. 205:320-326, 1994). Both proteins have phospho...
متن کاملDomain 2 of nonstructural protein 5A (NS5A) of hepatitis C virus is natively unfolded.
Nonstructural protein 5A protein (NS5A) of hepatitis C virus (HCV) plays an important role in the regulation of viral replication, interferon resistance, and apoptosis. HCV NS5A comprises three domains. Recently the structure of domain 1 has been determined, revealing a structural scaffold with a novel zinc-binding motif and a disulfide bond. At present, the structures of domains 2 and 3 remain...
متن کاملRegulation of mRNA translation and cellular signaling by hepatitis C virus nonstructural protein NS5A.
The NS5A nonstructural protein of hepatitis C virus (HCV) has been shown to inhibit the cellular interferon (IFN)-induced protein kinase R (PKR). PKR mediates the host IFN-induced antiviral response at least in part by inhibiting mRNA translation initiation through phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha). We thus examined the effect of NS5A inhibition ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1995
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.69.7.3980-3986.1995